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5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N2-isobutyryl

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5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N2-isobutyryl

Name: 5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N2-isobutyryl
Brand: A. B. ENTERPRISES
Price: 12300 INR
Availability: InStock

Price 12300 INR/ Kilograms

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MOQ : 5 Grams

5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N2-isobutyryl Specification

Ingredients
5-O-(4,4-dimethoxytrityl), 2-O-[(1,1-dimethylethyl)-dimethylsilyl], N2-isobutyryl protected nucleoside

Shelf Life
2 years under recommended storage

Molecular Weight
780.03 g/mol

Purity
>98% (HPLC)

Smell
Odorless

Product Type
Nucleoside phosphoramidite derivative

Shape
Powder

Usage
For research and laboratory use in automated DNA/RNA synthesizers

Grade
Research grade

Appearance
White to off-white crystalline powder

Structural Formula
Refer to product image for detailed structure

Physical Form
Solid (powder)

Properties
Moisture sensitive, air sensitive, for research and synthesis

Storage
Store at -20°C in a dry and inert atmosphere

Application
Oligonucleotide synthesis, RNA synthesis with 2-O-protected nucleoside

Molecular Formula
C44H56N4O7Si

Poisonous
No (for laboratory use only)

HS Code
29349900

Solubility
Soluble in acetonitrile, dichloromethane; insoluble in water

Melting Point
n/a (decomposes)

Handling Precautions
Use under dry, inert conditions; wear gloves and protective equipment

Analysis Method
HPLC, NMR, MS (Certificate of Analysis available)

Packaging
Sealed amber glass vial with inert gas

Synonyms
DMTr-TBS-N2-isobutyryl protected guanosine

Protecting Groups
5'-DMTr, 2'-TBS, N2-isobutyryl

Stability
Stable under anhydrous conditions

Sensitive to
Moisture, acids, bases, light

Product Code
5O44DMT2OTBSDMN2IBU

End Use
Building block for chemical RNA synthesis

5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N2-isobutyryl Trade Information

Minimum Order Quantity
5 Grams

Supply Ability
100 Grams Per Week

Delivery Time
1 Week

Main Export Market(s)
Australia, North America, South America, Eastern Europe, Western Europe, Middle East, Africa, Central America, Asia

Main Domestic Market
All India

About 5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N2-isobutyryl

5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N2-isobutyryl

CAS No : [147201-04-5]

M.Wt. 769.95

M.F. C41H51N5O8Si

Specification :

*Appearance: White to Off white powder,

*HPLC Purity: NLT 98%

*5'-ODMT-2'-OTBDMS Uridine (3'-Isomer): NMT 0.3%

*Residual Solvent: NMT 3.0%

*Moisture content by KF: NMT 1.0%

*1H NMR (Solvent : DMSO d6): Conforms with Structure

*LCMS: Corresponds to Mass

*APHA Test in 0.2M in DMSO: NMT 250

*Solubility: 0.2M in MDC

Advanced Building Block for RNA Synthesis

This protected guanosine derivative is expertly formulated for precision in automated laboratory oligonucleotide synthesis. With its multiple protection groups-5-DMTr, 2-TBS, and N2-isobutyryl-it permits selective deprotection steps while preserving sensitive functionalities. Its high purity and protection against undesired reactions render it a reliable starting material for tailored RNA sequences, supporting research in molecular biology and synthetic chemistry.

Strict Handling and Storage Requirements

The compound's sensitivity to atmospheric moisture, acids, bases, and light necessitates strict precautions during storage and use. It should be handled only under dry, inert atmospheres such as argon or nitrogen, while wearing appropriate protective equipment. Proper storage at -20C in a sealed amber glass vial with inert gas significantly extends shelf life, maintaining compound quality for up to two years.

FAQ's of 5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N2-isobutyryl:

Q: How should 5-O-(4,4-dimethoxytrityl) 2-O-[(1,1-dimethylethyl)-dimethylsilyl] N2-isobutyryl protected guanosine be handled and stored?

A: This compound should always be manipulated in a dry, inert atmosphere, such as a glove box or under nitrogen/argon. Store it at -20C, sealed in its original amber glass vial under inert gas, to prevent degradation from moisture, air, acids, bases, or light.

Q: What is the primary use of DMTr-TBS-N2-isobutyryl protected guanosine?

A: Its main application is as a protected guanosine building block for chemical RNA synthesis, especially in automated DNA/RNA synthesizers for research laboratory purposes.

Q: When do I need to use this specific protected guanosine over unprotected alternatives?

A: You should use this protected form when precise protection of the 5', 2', and N2 positions of guanosine is required to achieve high selectivity and yield in oligonucleotide synthesis, especially when synthesizing RNA with 2'-O-protected nucleosides.

Q: Where is this product typically used or integrated in the research process?

A: This compound is typically used during the solid-phase chemical synthesis of RNA oligonucleotides, serving as a key monomer incorporated by automated synthesizers to ensure sequence accuracy and integrity.

Q: What are the benefits of using a research-grade, high-purity guanosine building block?

A: Using a research-grade compound with >98% purity, like this, reduces the risk of impurities interfering with synthesis, thereby enhancing the efficiency and consistency of RNA assembly, leading to more reliable experimental results.

Q: How is product quality confirmed before dispatch?

A: Quality is validated using HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis accompanies each batch to assure researchers of purity, identity, and compliance with specified standards.

Q: Is there a recommended process for dissolving this compound for synthesis?

A: Yes, dissolve the compound in dry acetonitrile or dichloromethane under an inert atmosphere just prior to use. Water must be excluded to maintain its stability and reactivity during oligonucleotide synthesis.

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