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Nitro blue tetrazolium chloride

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MOQ : 50 Kilograms

Nitro blue tetrazolium chloride Specification

Product Type
Analytical Reagent, Biochemical Reagent

Shelf Life
24 months (unopened, proper storage)

Shape
Crystalline powder

Storage
Store at 2-8C, protect from light and moisture

Melting Point
~260C (decomposes)

Solubility
Soluble in water, DMSO, formamide

Application
Biological staining, enzymatic detection, diagnostic tests, detection of alkaline phosphatase activity

Smell
Odorless

Grade
Laboratory Grade

Molecular Formula
C40H30Cl2N10O6

Molecular Weight
817.64 g/mol

Ph Level
Neutral

Poisonous
YES

Usage
For research and laboratory use only

Physical Form
Powder

Properties
Oxidizing, water soluble, sensitive to light

Ingredients
Nitro blue tetrazolium chloride

Structural Formula
[C40H30Cl2N10O6]

EINECS No
206-067-4

CAS No
298-83-9

Taste
Not determined

HS Code
29349900

Purity
>98%

Appearance
Dark purple solid

Nitro blue tetrazolium chloride Trade Information

Minimum Order Quantity
50 Kilograms

Supply Ability
1 , , Kilograms Per Week

Delivery Time
1 Week

About Nitro blue tetrazolium chloride

Nitro blue tetrazolium chloride

CAS Number : 298-83-9

Molecular Formula : C40H30N10O6Cl2

Molecular Weight(Mass) : 817.65gm/mole

Appearance : Yellow crystalline powder

Assay(ex Cl,anhydrous): 95%

Solubility : 5% methanolic solution is clear and yellow

Versatile Applications

Nitro blue tetrazolium chloride serves as a crucial reagent in laboratory processes, particularly for biological staining and diagnostic tests. Its main benefit lies in its ability to detect enzymatic activity, such as alkaline phosphatase, providing reliable results for research and diagnostics.

Safe Handling and Storage

This compound should be handled with caution due to its harmful effects if swallowed. Always wash hands thoroughly after use and store the product in a cool environment (2-8C), shielded from light and moisture, to maintain stability and preserve its efficacy.

Chemical and Physical Properties

Appearing as a dark purple, odorless crystalline powder, NBT is highly soluble in water and dimethyl sulfoxide. It is sensitive to light and decomposes at elevated temperatures rather than melting, making appropriate storage essential for safety and longevity.

FAQs of Nitro blue tetrazolium chloride:

Q: How should Nitro blue tetrazolium chloride be stored to maintain its stability?

A: Nitro blue tetrazolium chloride should be stored at 2-8C in a moisture-free area, protected from light, as it is sensitive to light exposure. Proper storage ensures a shelf life of up to 24 months when unopened.

Q: What are the primary uses of Nitro blue tetrazolium chloride in laboratory research?

A: NBT is utilized for biological staining, enzymatic detection, diagnostic testing, and specifically for detecting alkaline phosphatase activity in research and laboratory environments.

Q: When is it necessary to use compatible solvents with Nitro blue tetrazolium chloride?

A: Compatible solvents like water and dimethyl sulfoxide (DMSO) should be used when preparing solutions of NBT for laboratory and research applications to ensure proper solubility and reaction performance.

Q: Where can Nitro blue tetrazolium chloride be acquired for laboratory use?

A: This chemical is available from distributors, exporters, manufacturers, suppliers, traders, retailers, and wholesalers in India, with various grades and purities offered depending on laboratory requirements.

Q: What precautions should be taken during the handling process of Nitro blue tetrazolium chloride?

A: Handling NBT requires thorough washing of hands after use, as it is harmful if swallowed. Additionally, always use protective equipment and follow safety protocols to mitigate exposure risks.

Q: What is the benefit of using Nitro blue tetrazolium chloride in enzymatic detection assays?

A: NBT provides reliable colorimetric results that facilitate the detection of enzyme activity, particularly alkaline phosphatase, which aids researchers in accurate and efficient diagnostic analyses.

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