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Nitro blue tetrazolium chloride

Name: Nitro blue tetrazolium chloride
Brand: A. B. ENTERPRISES

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MOQ : 50 Kilograms

Nitro blue tetrazolium chloride Specification

Properties
Oxidizing, water soluble, sensitive to light

Purity
>98%

Molecular Formula
C40H30Cl2N10O6

Solubility
Soluble in water, DMSO, formamide

Physical Form
Powder

Smell
Odorless

CAS No
298-83-9

Taste
Not determined

Usage
For research and laboratory use only

Product Type
Analytical Reagent, Biochemical Reagent

HS Code
29349900

Grade
Laboratory Grade

Molecular Weight
817.64 g/mol

Application
Biological staining, enzymatic detection, diagnostic tests, detection of alkaline phosphatase activity

Structural Formula
[C40H30Cl2N10O6]

Shelf Life
24 months (unopened, proper storage)

Storage
Store at 2-8C, protect from light and moisture

Poisonous
YES

Ph Level
Neutral

Ingredients
Nitro blue tetrazolium chloride

EINECS No
206-067-4

Shape
Crystalline powder

Appearance
Dark purple solid

Melting Point
~260C (decomposes)

Stability
Stable under recommended conditions

Precautionary Statements
P264: Wash hands thoroughly after handling

UN Number
Not regulated

Light Sensitivity
Sensitive to light, store protected from light

Compatible Solvents
Water, dimethyl sulfoxide (DMSO)

Boiling Point
Not applicable, decomposes

EC Number
206-067-4

Synonyms
Nitroblue tetrazolium, NBT

Hazard Statements
H302: Harmful if swallowed

Nitro blue tetrazolium chloride Trade Information

Minimum Order Quantity
50 Kilograms

Supply Ability
1 , , Kilograms Per Week

Delivery Time
1 Week

About Nitro blue tetrazolium chloride

Nitro blue tetrazolium chloride

CAS Number : 298-83-9

Molecular Formula : C40H30N10O6Cl2

Molecular Weight(Mass) : 817.65gm/mole

Appearance : Yellow crystalline powder

Assay(ex Cl,anhydrous): 95%

Solubility : 5% methanolic solution is clear and yellow

Versatile Applications

Nitro blue tetrazolium chloride serves as a crucial reagent in laboratory processes, particularly for biological staining and diagnostic tests. Its main benefit lies in its ability to detect enzymatic activity, such as alkaline phosphatase, providing reliable results for research and diagnostics.

Safe Handling and Storage

This compound should be handled with caution due to its harmful effects if swallowed. Always wash hands thoroughly after use and store the product in a cool environment (2-8C), shielded from light and moisture, to maintain stability and preserve its efficacy.

Chemical and Physical Properties

Appearing as a dark purple, odorless crystalline powder, NBT is highly soluble in water and dimethyl sulfoxide. It is sensitive to light and decomposes at elevated temperatures rather than melting, making appropriate storage essential for safety and longevity.

FAQs of Nitro blue tetrazolium chloride:

Q: How should Nitro blue tetrazolium chloride be stored to maintain its stability?

A: Nitro blue tetrazolium chloride should be stored at 2-8C in a moisture-free area, protected from light, as it is sensitive to light exposure. Proper storage ensures a shelf life of up to 24 months when unopened.

Q: What are the primary uses of Nitro blue tetrazolium chloride in laboratory research?

A: NBT is utilized for biological staining, enzymatic detection, diagnostic testing, and specifically for detecting alkaline phosphatase activity in research and laboratory environments.

Q: When is it necessary to use compatible solvents with Nitro blue tetrazolium chloride?

A: Compatible solvents like water and dimethyl sulfoxide (DMSO) should be used when preparing solutions of NBT for laboratory and research applications to ensure proper solubility and reaction performance.

Q: Where can Nitro blue tetrazolium chloride be acquired for laboratory use?

A: This chemical is available from distributors, exporters, manufacturers, suppliers, traders, retailers, and wholesalers in India, with various grades and purities offered depending on laboratory requirements.

Q: What precautions should be taken during the handling process of Nitro blue tetrazolium chloride?

A: Handling NBT requires thorough washing of hands after use, as it is harmful if swallowed. Additionally, always use protective equipment and follow safety protocols to mitigate exposure risks.

Q: What is the benefit of using Nitro blue tetrazolium chloride in enzymatic detection assays?

A: NBT provides reliable colorimetric results that facilitate the detection of enzyme activity, particularly alkaline phosphatase, which aids researchers in accurate and efficient diagnostic analyses.

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