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5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N4-acetyl Cytidine

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5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N4-acetyl Cytidine

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5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N4-acetyl Cytidine Specification

Melting Point
195-205°C (decomposes)

Solubility
Soluble in dichloromethane (DCM), acetonitrile, DMF, and other organic solvents. Insoluble in water.

Poisonous
Non-poisonous for laboratory handling, avoid ingestion/inhalation.

Appearance
Off-white to pale yellow powder

Molecular Formula
C41H50N4O9Si

Ingredients
5-O-(4,4-dimethoxytrityl) 2-O-[(1,1-dimethylethyl)-dimethylsilyl] N4-acetyl Cytidine

Application
Phosphoramidite chemistry, solid phase oligonucleotide/DNA synthesis.

CAS No
148015-95-6

Shape
Amorphous solid

Molecular Weight
770.96 g/mol

Physical Form
Solid

HS Code
29349900

Taste
Not determined

Density
Gram per cubic centimeter(g/cm3)

Properties
Protecting group: 5-O-(4,4-dimethoxytrityl), 2-O-TBDMS, N4-acetyl. High chemical purity suitable for oligonucleotide synthesis. Moisture sensitive.

Purity
>98% (HPLC)

Product Type
Nucleoside derivative

Storage
Store at -20°C, desiccated, protected from light and moisture.

Shelf Life
12 months under proper storage conditions.

Smell
Odorless

Grade
Analytical/Research grade

Structural Formula
Refer to product-specific image or chemical drawing.

Usage
Used in automated DNA/RNA synthesizers for introduction of protected cytidine residues.

IUPAC Name
N4-acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)cytidine

Stability
Stable under recommended storage conditions, sensitive to acids, bases, and moisture.

Hazard Statements
Not classified as hazardous under GHS for laboratory use.

Transport
Not regulated as dangerous goods for transport.

Packaging
Supplied in amber glass vials sealed under inert gas.

Handling Precautions
Use gloves, work under dry and inert atmosphere if possible.

Protecting Groups
5'-O-Dimethoxytrityl, 2'-O-tert-butyldimethylsilyl, N4-acetyl

Synonyms
DMTr-TBDMS-N4-acetyl-cytidine

5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N4-acetyl Cytidine Trade Information

Supply Ability
100 Per Week

Delivery Time
1 Week

Main Export Market(s)
Australia, North America, South America, Eastern Europe, Western Europe, Middle East, Africa, Central America, Asia

Main Domestic Market
All India

About 5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N4-acetyl Cytidine

5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N4-acetyl Cytidine,

CAS No : [121058-85-3]

M.Wt. 701.88

M.F. C38H47N3O8Si

Specification :

*Appearance: White to Off white powder,

*HPLC Purity: NLT 98%

*5'-ODMT-2'-OTBDMS Uridine (3'-Isomer): NMT 0.3%

*Residual Solvent: NMT 3.0%

*Moisture content by KF: NMT 1.0%

*1H NMR (Solvent : DMSO d6): Conforms with Structure

*LCMS: Corresponds to Mass

*APHA Test in 0.2M in DMSO: NMT 250

*Solubility: 0.2M in MDC

Applications in Oligonucleotide Synthesis

This protected cytidine derivative is specifically formulated for use in phosphoramidite chemistry, facilitating the solid-phase synthesis of DNA and RNA. Its application streamlines the introduction of cytidine bases in automated synthesizers, ensuring efficient and high-fidelity oligonucleotide assembly. High purity and well-chosen protecting groups make it ideal for producing modified nucleic acids in research and biotechnology settings.

Optimal Storage and Handling

For maximum stability, store the compound at -20C in a desiccator, shielded from light and humidity. Due to its sensitivity to acids, bases, and moisture, handle it with gloves and, when possible, under an inert atmosphere. Supplied in amber glass vials sealed under inert gas, the product maintains integrity for up to 12 months. Always follow standard laboratory safety measures during use.

FAQ's of 5'-O-(4,4'-dimethoxytrityl) 2'-O-[(1,1-dimethylethyl)-dimethylsilyl] N4-acetyl Cytidine:

Q: How should 5-O-(4,4-dimethoxytrityl) 2-O-[(1,1-dimethylethyl)-dimethylsilyl] N4-acetyl Cytidine be stored?

A: This compound should be stored at -20C in a well-sealed container, protected from moisture and light, preferably under an inert atmosphere. Desiccation is recommended to prevent degradation.

Q: What is the primary usage of this protected cytidine derivative?

A: It is primarily employed in automated DNA and RNA synthesizers as a building block for the introduction of protected cytidine residues during phosphoramidite chemistry-based oligonucleotide assembly.

Q: When is it necessary to work under an inert atmosphere with this product?

A: To preserve its chemical stability and prevent moisture-induced degradation, it is advisable to handle the product under a dry, inert atmosphere, especially during weighing and transfer processes.

Q: Where does this compound find practical application?

A: The compound is widely used in research laboratories and biotechnology facilities specializing in custom oligonucleotide synthesis for molecular biology, diagnostics, and therapeutic investigations.

Q: What benefits do the specific protecting groups provide in this product?

A: The 5-O-dimethoxytrityl, 2-O-tert-butyldimethylsilyl, and N4-acetyl groups safeguard reactive functionalities on the cytidine nucleoside, enabling sequential and selective deprotection during oligonucleotide synthesis and ensuring high coupling efficiency.

Q: How does the product's solubility profile affect its processing?

A: Being soluble in organic solvents such as dichloromethane, acetonitrile, and DMF, but insoluble in water, the product integrates smoothly into organic-phase reactions commonly used in oligonucleotide synthesis.

Q: What are the safety and hazard considerations for handling this compound?

A: The product is not classified as hazardous for laboratory use. Nonetheless, avoid ingestion or inhalation and always use personal protective equipment, such as gloves. There are no special transport regulations, and it is considered non-poisonous under standard handling guidelines.

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