Product Description
Coomassie Brilliant Blue R 250 is a dark blue, odorless, and tasteless powder used for staining proteins in gel electrophoresis, primarily for SDS-PAGE and native gel protein visualization. With a purity level of over 99% and a molecular weight of 825.97 g/mol, this laboratory-grade dye exhibits a strong binding affinity for proteins. It is soluble in water and methanol and should be stored in a cool, dry place away from light for a shelf life of 2 years. Its molecular formula C45H44N3NaO7S2 includes sulfonic and amine groups, and it is available in granular or fine powdered form.
FAQs of COOMASIE BRILLIANT BLUE R 250 For GEL Electrophoresis:
Q: What is the primary application of Coomassie Brilliant Blue R 250?
A: Coomassie Brilliant Blue R 250 is primarily used for staining proteins in gel electrophoresis, specifically for SDS-PAGE and native gel protein visualization.
Q: How should Coomassie Brilliant Blue R 250 be stored?
A: It should be stored in a cool, dry place away from light to ensure its stability and maintain its properties for up to 2 years.
Q: Is Coomassie Brilliant Blue R 250 soluble in water?
A: Yes, Coomassie Brilliant Blue R 250 is soluble in both water and methanol, making it convenient for laboratory applications.
Q: What is the molecular weight of Coomassie Brilliant Blue R 250?
A: The molecular weight of Coomassie Brilliant Blue R 250 is 825.97 g/mol, indicating its mass in atomic mass units.
Q: Can Coomassie Brilliant Blue R 250 be used for any other applications apart from gel electrophoresis?
A: While its primary use is in gel electrophoresis for protein staining, it can also be applied in other laboratory settings for various purposes.