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5'-ODMT N-Bz dC (PNS)

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5'-ODMT N-Bz dC (PNS) Specification

Usage
Used as a protected cytidine phosphoramidite in automated DNA synthesis

Solubility
Soluble in acetonitrile, dichloromethane, and DMF

Ingredients
5-O-DMT-N4-Benzoyl-2-deoxycytidine-3-O-(2-cyanoethyl N,N-diisopropylphosphoramidite)

Properties
Protected deoxycytidine for DNA synthesis, high purity, stable under recommended storage conditions

Density
Gram per cubic centimeter(g/cm3)

Product Type
Nucleoside Phosphoramidite

Refractive Rate
Not determined

Purity
>98% (HPLC)

Physical Form
Powder

Shelf Life
12 months under recommended storage

Shape
Crystalline

Appearance
Off-white to white crystalline powder

Taste
Not determined

Grade
Molecular Biology Grade

HS Code
29349900

Melting Point
Not applicable/Decomposes

Structural Formula
[Refer to structural diagram of 5-O-DMT-N-Bz-dC (PNS)]

Molecular Weight
792.86 g/mol

CAS No
98796-52-2

Molecular Formula
C43H49N6O7P

Application
DNA/RNA oligonucleotide synthesis

Smell
Odorless

Poisonous
Non-poisonous

Storage
-20°C, desiccated and protected from light

Synonyms
5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite

Handling Precautions
Avoid contact with moisture; handle under inert atmosphere

Packing
Sealed amber glass vials

Compatible Synthesizers
All standard DNA/RNA synthesizers

Stability
Stable for 12 months at -20°C in original unopened container

Typical Impurities
<2% (Related compounds)

5'-ODMT N-Bz dC (PNS) Trade Information

Supply Ability
100 Per Week

Delivery Time
1 Week

Main Export Market(s)
Australia, Eastern Europe, Central America, Middle East, South America, Western Europe, Asia, North America, Africa

Main Domestic Market
All India

About 5'-ODMT N-Bz dC (PNS)

5'-ODMT N-Bz dC (PNS)

CAS No : [67219-55-0]

M.Wt. 633.7

M.F.C37H35N3O7

Specification :

*Appearance: White to Off White Powder

*Purity by HPLC (%Area): NLT 98.0%

*1H NMR (Solvent:DMSO d6): Conforms with structure

*LCMS: Corresponds to Mass

*Moisture Content (KF w/w): NMT 1.0%

*Residual Solvents (%w/w): NMT 3.0%

*APHA in 0.2M DMSO solution: NMT 300

*Solubility: 0.2M in DMF

Advanced Nucleoside Protection for DNA Synthesis

5-ODMT N-Bz dC (PNS) provides stable protection to the cytidine base, ensuring reliable and efficient incorporation during automated DNA oligonucleotide synthesis. Its advanced protecting groups and high purity make it suitable for the sensitive requirements of molecular biology research, assisting in the generation of high-fidelity synthetic DNA strands.

Optimal Storage and Stability

This phosphoramidite demonstrates robust stability when kept at -20C in its original packaging, protected from moisture and light. Its shelf life extends up to 12 months under these recommended conditions, offering convenience and reliable performance across multiple synthesis batches.

Versatility and Compatibility

Compatible with all major DNA/RNA synthesizers, 5-ODMT N-Bz dC (PNS) integrates smoothly into standard oligonucleotide synthesis workflows. Its solubility in organic solvents like acetonitrile and DMF ensures effective reagent preparation and use in various synthesis protocols.

FAQ's of 5'-ODMT N-Bz dC (PNS):

Q: How should 5-ODMT N-Bz dC (PNS) be stored for optimal stability?

A: To ensure maximum stability and shelf life, store the product at -20C in its original, unopened amber glass vial. Keep it desiccated and protected from light to prevent degradation.

Q: What is the primary application of 5-ODMT N-Bz dC (PNS) in the laboratory?

A: This reagent is mainly used as a protected cytidine phosphoramidite during automated DNA oligonucleotide synthesis, enabling precise and efficient sequence construction in research and diagnostic settings.

Q: When can I expect the reagent to remain stable and effective for use?

A: 5-ODMT N-Bz dC (PNS) retains its stability and high purity for up to 12 months if stored under the recommended conditions in a tightly sealed container at -20C.

Q: Where can 5-ODMT N-Bz dC (PNS) be used?

A: It is suitable for use in laboratories utilizing automated DNA/RNA synthesizers, including academic, industrial, and commercial molecular biology facilities worldwide.

Q: What precautions are necessary when handling this phosphoramidite?

A: Avoid exposure to moisture and handle the product under an inert atmosphere to maintain its quality. Use protective equipment to prevent skin or eye contact.

Q: What advantages does this reagent offer compared to unprotected cytidine?

A: Its protective groups minimize side reactions during synthesis, delivering high-purity, well-defined oligonucleotides ideal for sensitive molecular biology applications.

Q: How is 5-ODMT N-Bz dC (PNS) typically processed during DNA synthesis?

A: The reagent is dissolved in a compatible organic solvent such as acetonitrile and loaded onto a standard DNA/RNA synthesizer where it participates in stepwise oligonucleotide chain elongation.

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