About BROMO PHENOL BLUE
Backed by a team of skilled and trained professionals, we are able to provide the clients with BROMO PHENOL BLUE. These products are used by varied industries as acid-base indicator, color marker and dye. Owing to our advanced techniques and modern machinery, we are able ensure the non-contaminated form of these chemicals. Prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid, these products have a pure and accurate composition. BROMO PHENOL BLUE is available with us in different quantities in accordance to the needs of the clients.
Features:
- Acid based
- Moderate pH value
- Longer shelf life
- High usage
- Color changing ability
Specification Sheet
| CAS No. | 115 - 39 - 9 |
| Chemical Formula | C19H10Br4O5S |
| Molecular Weight | 669.98 |
| Melting Point | 272 - 273o C |
| Dye Content | 5% |
| Transition Range | pH 3.0 - 4.6 Greenish Yellow - Reddish Blue |
| Absorption Max (pH3.0) | 434 - 439 nm |
| Absorption Max (pH4.6) | 590 - 593 nm |
| Absorptivity (1%,1cm) pH 3.0 | 350 - 385 |
| Absorptivity (1%,1cm) pH 4.6 | 940 - 1000 |
| Loss On Drying (110o C) | <1% |
Versatile Laboratory ReagentBromophenol Blue serves as a reliable and multi-purpose laboratory indicator, dye, and reagent. Due to its minimum purity level of 98%, it is well-suited for high-precision analytical work and is commonly adopted in molecular biologyparticularly in electrophoresis to track sample movement. Its color-changing properties provide clear, visual cues about pH changes, aiding in various chemical and biological experiments.
Stable and Convenient PackagingTo maintain its integrity and stability, Bromophenol Blue is packaged in robust plastic bottles, glass bottles, or drums. These packaging options protect the compound from environmental factors, ensuring a consistent product over its extended five-year shelf life. The availability of multiple packaging sizes25 g, 100 g, 500 g, and 1 kgcaters to both small-scale experiments and larger industrial or research applications.
FAQs of BROMO PHENOL BLUE:
Q: How is Bromophenol Blue commonly used in laboratory settings?
A: Bromophenol Blue is used as a pH indicator, laboratory reagent, and tracking dye in electrophoresis. Its color change from yellow (pH 3.0) to purple (pH 4.6) makes it particularly useful for identifying pH changes, while its visibility helps in monitoring sample progress in gel-based separation techniques.
Q: What benefit does the high purity (98%) of Bromophenol Blue offer?
A: A high purity level of at least 98% ensures that Bromophenol Blue delivers consistent and reliable performance in sensitive laboratory applications. This reduces the likelihood of interference from contaminants, resulting in more accurate experimental outcomes.
Q: When should Bromophenol Blue be replaced or disposed of?
A: Bromophenol Blue has a shelf life of five years when stored under recommended conditions. It should be replaced once this period has elapsed or if the product shows signs of contamination or degradation, such as clumping or discoloration.
Q: Where is Bromophenol Blue available for purchase in India?
A: Bromophenol Blue is widely accessible in India through various channels as it is stocked by distributors, exporters, importers, manufacturers, retailers, suppliers, traders, and wholesalers. It is available in packaging sizes ranging from 25 g to 1 kg.
Q: What is the process for using Bromophenol Blue as a pH indicator?
A: To use Bromophenol Blue as a pH indicator, dissolve the powder in distilled water to the desired concentration. Apply a small volume to your sample solution; the dye will shift from yellow at pH 3.0 to purple at pH 4.6, indicating the pH range.
Q: What precautions are necessary for handling and storing Bromophenol Blue?
A: Store Bromophenol Blue in tightly sealed containers made of plastic, glass, or drums in a cool, dry environment away from direct sunlight. Proper handling avoids degradation, ensuring its stability and reliable performance throughout its shelf life.